C-terminal short arginine/serine repeat sequence-dependent regulation of Y14 (RBM8A) localization.
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Abstract |
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Y14 (RBM8A) is an RNA recognition motif-containing protein that forms heterodimers with MAGOH and serves as a core factor of the RNA surveillance machinery for the exon junction complex (EJC). The role of the Y14 C-terminal serine/arginine (RS) repeat-containing region, which has been reported to undergo modifications such as phosphorylation and methylation, has not been sufficiently investigated. Thus, we aimed to explore the functional significance of the Y14 C-terminal region. Deletion or dephosphorylation mimic mutants of the C-terminal region showed a shift in localization from the nucleoplasmic region; in addition, the C-terminal RS repeat-containing sequence itself exhibited the potential for nucleolar localization. Additionally, the regulation of Y14 localization by the C-terminal region was further found to be exquisitely controlled by MAGOH binding. Cumulatively, our findings, which demonstrated that Y14 localization is regulated not only by the previously reported N-terminal localization signal but also by the C-terminal RS repeat-containing region through phosphorylation and MAGOH binding to Y14, provide new insights for the mechanism of localization of short RS repeat-containing proteins. |
Year of Publication |
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2018
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Journal |
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Scientific reports
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Volume |
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8
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Issue |
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1
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Number of Pages |
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612
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Date Published |
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2018
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URL |
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http://dx.doi.org/10.1038/s41598-017-18765-1
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DOI |
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10.1038/s41598-017-18765-1
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Short Title |
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Sci Rep
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