Ribozyme-mediated trans insertion-splicing into target RNAs.
| Author | |
| Abstract |
:
The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction. |
| Year of Publication |
:
0
|
| Journal |
:
Methods in molecular biology (Clifton, N.J.)
|
| Volume |
:
848
|
| Number of Pages |
:
385-94
|
| Date Published |
:
2012
|
| ISSN Number |
:
1064-3745
|
| URL |
:
https://dx.doi.org/10.1007/978-1-61779-545-9_24
|
| DOI |
:
10.1007/978-1-61779-545-9_24
|
| Short Title |
:
Methods Mol Biol
|
| Download citation |
