Study of key control points in ripening is essential to improve grape wine quality. Molecular basis of ripening is still far from being understood from the Pierce's disease tolerant grapes predominantly grown in the Southeastern United States. To identify proteins expressed during Blanc Du Bois grape berry green and ripening stages, proteome analysis from five different stages revealed 1091, 1131, 1078, 1042 and 1066 proteins. Differential expression analysis revealed 551 common proteins across different stages of maturity that are involved in various biochemical and metabolic pathways. The proteins identified were associated with phenylpropanoids, isoquinoline alkaloids, fatty acids, unsaturated fatty acids, and furanones. Our data provides the first step to understand the complex biochemical changes during ripening of Pierce's disease tolerant American hybrid grapes that are popular for their aroma and flavor profile in the southeastern USA. Proteomics data is deposited to the ProteomeXchangePXD004157. This article is protected by copyright. All rights reserved.