Efficient construction of xenogeneic genomic libraries by circumventing restriction-modification systems that restrict methylated DNA.
| Author | |
|---|---|
| Abstract |
:
An efficient method to construct xenogeneic genomic libraries with low errors and bias by circumventing restriction-modification systems that restrict methylated DNA was developed. Un-methylated genomic DNA of Escherichia coli prepared by ϕ29 DNA polymerase was introduced to Corynebacterium glutamicum R after ligation with un-methylated vector plasmids. |
| Year of Publication |
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2018
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| Journal |
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Journal of microbiological methods
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| Date Published |
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2018
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| ISSN Number |
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0167-7012
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| URL |
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http://linkinghub.elsevier.com/retrieve/pii/S0167-7012(18)30009-5
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| DOI |
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10.1016/j.mimet.2018.01.005
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| Short Title |
:
J Microbiol Methods
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| Download citation |
